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Search: Posts Made By: wanadou
Forum: Bioinformatics 02-10-2019, 02:21 AM
Replies: 1
Views: 906
Posted By wanadou
Fastq.gz file split

Hi,
I need to split a Fastq.gz file (2.8 Gb) in small files with R or others soft in windows7, 64bit.
thx
Forum: Bioinformatics 01-27-2019, 08:02 PM
Replies: 1
Views: 1,040
Posted By wanadou
NGS data analysis

I am beginner in the field of Bioinformatics
I received HiSeq illuminina 100 pb PE sequencing clean data as a fastq file
what are the steps to successfully assemble this genome of 400 MB?
It is...
Forum: RNA Sequencing 07-31-2018, 01:32 AM
Replies: 0
Views: 983
Posted By wanadou
plant transcription factor enrichement

How to find plant transcription factors enriched in a list of genes?
Forum: Bioinformatics 03-14-2018, 11:16 PM
Replies: 13
Views: 2,190
Posted By wanadou
Hello nucacidhunter, Thanks for your reply ...

Hello nucacidhunter,
Thanks for your reply
The cost of sequencing using PacBio is relatively high. the sequencing of 1 cell SMRT can give 5Gb. I think to do an preliminary assembly for a draft...
Forum: Bioinformatics 03-13-2018, 10:29 PM
Replies: 13
Views: 2,190
Posted By wanadou
help for NGS and assembly

Hello

I think to do the whole genome sequencing of a halophyte plant (350 Mbp) using the Hiseq Pe150 which generate data with 30X coverage
1- this strategy could it be effective to make an de...
Forum: Bioinformatics 02-25-2018, 10:22 PM
Replies: 6
Views: 2,261
Posted By wanadou
Thanks wkusmire and jgibbons1 for your reply I...

Thanks wkusmire and jgibbons1 for your reply
I have not yet started sequencing but I'm getting ready to do it.
Is it possible to recommend me a company to do it?
Forum: Bioinformatics 02-25-2018, 01:31 AM
Replies: 6
Views: 2,261
Posted By wanadou
help to do genome assembly

I prepare genomic shotgun libraries for sequencing using Single Molecule Real Time (SMRT) sequencing platform produced by PacBio (20X coverage of PacBio reads) coupled with sequencing on Illumina...
Forum: Bioinformatics 01-22-2018, 08:51 PM
Replies: 2
Views: 708
Posted By wanadou
Smile Thanks Genomax

Thanks Genomax
Forum: Bioinformatics 01-21-2018, 09:15 PM
Replies: 2
Views: 708
Posted By wanadou
Help for the performance needed for computer

Hi everybody,
I want to set up a computer to process the generated data for de novo genome sequencing (800 Mb) by Roche 454 and illuminate. What are the performance and / or features needed for this...
Forum: Introductions 01-21-2018, 08:56 PM
Replies: 0
Views: 990
Posted By wanadou
Smile Hello our next generation sequencing community

Hello our next generation sequencing community
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