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Forum: Illumina/Solexa 12-14-2016, 11:24 AM
Replies: 2
Views: 1,097
Posted By ox_zoo
slight over clustering?

Hello,

I have, this morning, set up a Next-Seq run for some ChIP-seq libraries. It is now on its 39th cycle. However, the cluster PF value is 75.94 yet the %≥Q30 is at 90.82. I have around...
Forum: Illumina/Solexa 11-19-2016, 06:48 AM
Replies: 6
Views: 1,818
Posted By ox_zoo
The cluster density for the four lanes range from...

The cluster density for the four lanes range from 0-250k/mm2.

The lanes:
Lane 1: 235 41
Lane 2: 237 38
Lane 3: 212 56
Lane 4: 230 35

I hope this helps.
Forum: Illumina/Solexa 11-19-2016, 06:37 AM
Replies: 6
Views: 1,818
Posted By ox_zoo
Thanks for your reply. Yes I will do that -...

Thanks for your reply.

Yes I will do that - unfortunately since its a weekend they cannot reply.

I was thinking - if clusters are not formed in those two particular areas, would the cameras...
Forum: Illumina/Solexa 11-19-2016, 05:12 AM
Replies: 6
Views: 1,818
Posted By ox_zoo
Yes, now it has finished and the QC is only 15.2....

Yes, now it has finished and the QC is only 15.2.

The machine also has a warning message on two of the lanes:

camera disabled:failed to detect clusters


Does this mean that the cameras...
Forum: Illumina/Solexa 11-18-2016, 12:56 PM
Replies: 6
Views: 1,818
Posted By ox_zoo
Low QC scores?

I have just set up my very first sequencing experiment on the Next-Seq 500. It is now 45 cycles into PE sequencing, however it seems that my QC scores are a bit low. I only have a % >= Q30%: 25.2% ...
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