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Forum: Metagenomics 10-16-2018, 06:55 AM
Replies: 5
Views: 5,902
Posted By ntremblay
Viral RNA was extracted from plasma samples using...

Viral RNA was extracted from plasma samples using QIAamp Viral RNA Mini Kit (720uL of sample) using standard protocol.

Double stranded cDNA synthesis was done using Maxima H Minus Double-Stranded...
Forum: Metagenomics 10-15-2018, 09:17 AM
Replies: 5
Views: 5,902
Posted By ntremblay
@m02846:68:000000000-c39d4:1:1101:14422:2079...

@m02846:68:000000000-c39d4:1:1101:14422:2079 1:n:0:1
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+...
Forum: Metagenomics 10-09-2018, 01:05 PM
Replies: 5
Views: 5,902
Posted By ntremblay
Question AAAAAA.. TTTTTTT.. heterodimers

Good evening all,

I have prepared (ds)cDNA from viral RNA extracted from plasma that was subsequently sequenced on a MiSEQ following standard library preparation.

When I got the reads back, I...
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