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Forum: RNA Sequencing 11-03-2017, 03:26 PM
Replies: 4
Views: 4,859
Posted By jwfoley
No, I'm concerned that having a lot of random...

No, I'm concerned that having a lot of random bases would invite mispriming and other molecular tomfoolery, plus it's a waste of sequencing cycles if you're using short reads, as we do. Oh, and...
Forum: RNA Sequencing 11-02-2017, 07:15 AM
Replies: 4
Views: 4,859
Posted By jwfoley
Both great questions. Well, first of...

Both great questions.



Well, first of all, it has nothing to do with streptavidin, which always confuses people, and that's why our diagram (fig. S1 in Supplemental File 1) just calls it a...
Forum: RNA Sequencing 11-01-2017, 09:24 AM
Replies: 4
Views: 4,859
Posted By jwfoley
Post Smart-3SEQ: a cheap, fast protocol that is sensitive enough for FFPE single cells

Our lab has just posted a preprint (https://www.biorxiv.org/content/early/2017/10/27/207340) about our new RNA-seq protocol, Smart-3SEQ. It combines the previous 3SEQ method for 3' digital gene...
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