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Forum: General 12-24-2018, 11:44 AM
Replies: 3
Views: 2,161
Posted By luc
Hi Kujin, to my knowledge the Lohman...

Hi Kujin,

to my knowledge the Lohman protocol should work, as is, on the iSeq - as any other Truseq library design. We do not have an iSeq however. Do you want to optimize it for paired-end...
Forum: General 12-20-2018, 08:24 PM
Replies: 3
Views: 2,161
Posted By luc
Could you point us to your complete protocol? ...

Could you point us to your complete protocol? There are multiple Tag-seq protocols out there.
Likely your first amplification was already failing.
Your PCR cycles shows two consecutive stages at...
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