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Forum: General 10-03-2014, 10:09 AM
Replies: 3
Views: 1,498
Posted By eds285
So you're saying what exactly? Pool size doesn't...

So you're saying what exactly? Pool size doesn't matter? Keep pool size equal at the minimum of 2 and the sequencing can take care of the rest?
Forum: General 10-01-2014, 09:32 PM
Replies: 3
Views: 1,498
Posted By eds285
Question Are unequal pool sizes for RNA-seq acceptable?

Background:
I want to find differentially expressed genes between wild type (WT) and knockout (KO) mice of mixed background using RNA-seq (Illumina HiSeq, 50M reads standard at my core). There will...
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