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Forum: Illumina/Solexa 01-17-2019, 12:41 PM
Replies: 1
Views: 917
Posted By falconpunch
Linker sequences for 384-sample NovaSeq setup?

Hi everyone! I'm talking with my core facility about doing a new custom-insert run on the NovaSeq (currently doing it on a HiSeq 2500 but want to switch for lower cost and more flexibility). The idea...
Forum: Illumina/Solexa 10-05-2018, 09:35 AM
Replies: 3
Views: 1,409
Posted By falconpunch
Anyone have rec's for fast-turnaround MiSeq service?

I'm currently generating about a plate of 16S samples every 2 months, prepping and mixing them myself, and submitting to my university core facility (who shall remain nameless...).

Everything is...
Forum: Illumina/Solexa 10-05-2018, 09:27 AM
Replies: 1
Views: 1,330
Posted By falconpunch
I haven't used Rockhopper yet, but I have done...

I haven't used Rockhopper yet, but I have done similar analysis of bacterial mRNA with HiSeq 2000 reads in Geneius (expensive software).

I assume the blue area represents read depth by genome...
Forum: Illumina/Solexa 10-05-2018, 09:00 AM
Replies: 13
Views: 4,578
Posted By falconpunch
If you're still wondering after a month... ...

If you're still wondering after a month...

Generally no, you should see a tight band with any "normal" 16S amplification. This is because you're amplifying one specific DNA segment (typically the...
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