SEQanswers

Go Back   SEQanswers > Search Forums
Reload this Page Search Results
Register FAQ Members List Calendar Search Today's Posts Mark Forums Read


Showing results 1 to 7 of 7
Search took 0.00 seconds. Search: Posts Made By: mhgseq
Forum: Bioinformatics 06-07-2016, 09:28 AM
Replies: 0
Views: 1,129
Posted By mhgseq
How to extract sequence from multiple position in sorted bam files

Hi All,

My research need to extract sequence from multiple position in sorted bam files.
I have the bam files sorted. I would like to extract and keep sequence from multiple positions according...
Forum: Ion Torrent 04-28-2016, 08:36 AM
Replies: 8
Views: 8,114
Posted By mhgseq
thanks a lot. Your heat cooled approach works...

thanks a lot. Your heat cooled approach works well. We get much better reads this time.
Forum: Ion Torrent 04-20-2016, 09:16 AM
Replies: 8
Views: 8,114
Posted By mhgseq
We do check the bioanalyzer, not a lot of large...

We do check the bioanalyzer, not a lot of large size shift. But we do see a lots of small variation in size (our amplicon is about 200bp (include primer and adaptor), we do see peak at 180-250bp in...
Forum: Ion Torrent 04-19-2016, 04:46 PM
Replies: 8
Views: 8,114
Posted By mhgseq
Thank you so much for the info. I am also do the...

Thank you so much for the info. I am also do the similar short amplicon ( ~100bp + primers+ adaptor). But for the loading, we load much higher concentration for OT2 (200 pM vs the recommend 26 pM), I...
Forum: Ion Torrent 04-19-2016, 03:07 PM
Replies: 8
Views: 8,114
Posted By mhgseq
Have you solve your probelm of <60bp short reads....

Have you solve your probelm of <60bp short reads.

I have similar problem with my fusion primer amplicon seq with Ion Torrent. Everything is fine in last several month with up to 16 barcode. When...
Forum: Bioinformatics 10-14-2015, 08:08 AM
Replies: 678
Views: 212,317
Posted By mhgseq
Thank you very much. After specify K in both...

Thank you very much. After specify K in both command, it works.
Forum: Bioinformatics 10-13-2015, 03:35 PM
Replies: 678
Views: 212,317
Posted By mhgseq
out of memory error

even with a very small ref and input file, I got the out of memory error. Would you check what is happen?


bbmap.sh in=~/Documents/align/test.fa out=~/Documents/align/outputu.sam

java...
Showing results 1 to 7 of 7

 


All times are GMT -8. The time now is 09:26 PM.

Contact Us - SEQanswers Home - Archive - Top

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO