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Forum: Illumina/Solexa 05-14-2014, 05:53 PM
Replies: 5
Views: 5,086
Posted By rwinegar
We usually are processing les than 10 libraries. ...

We usually are processing les than 10 libraries. In this case, we have found that manual normalization is both easier and gives more predictable cluster densities than the bead normalization...
Forum: Bioinformatics 03-05-2014, 07:42 PM
Replies: 3
Views: 1,239
Posted By rwinegar
If you don't mind submitting your sequence to a...

If you don't mind submitting your sequence to a 3rd party, RAST is very straightforward
Forum: Sample Prep / Library Generation 07-26-2013, 10:19 AM
Replies: 9
Views: 11,081
Posted By rwinegar
When you use 100 pg input with XT do you scale...

When you use 100 pg input with XT do you scale back the volume or the amount of ATM or is the DNA quantity the only thing you change? Thanks so much.
Forum: Sample Prep / Library Generation 07-20-2013, 12:25 PM
Replies: 10
Views: 7,415
Posted By rwinegar
Great. Thanks so much. I may have more questions...

Great. Thanks so much. I may have more questions once we start working our way through this.
Forum: Sample Prep / Library Generation 07-20-2013, 12:13 PM
Replies: 10
Views: 7,415
Posted By rwinegar
Mcnelson, thanks for the useful information. ...

Mcnelson, thanks for the useful information. We've been considering dropping bead normalization because of variable clustering density. I was wondering how you quant the post PCR cleanup. Do you...
Forum: Illumina/Solexa 07-17-2013, 09:59 AM
Replies: 24
Views: 12,755
Posted By rwinegar
Unfortunately, we have encountered some similar...

Unfortunately, we have encountered some similar situations recently where there seems to be inhibitory factors in our samples. We always get terrific results from pure samples isolated with DNeasy,...
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