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Forum: Sample Prep / Library Generation 04-10-2012, 07:05 AM
Replies: 1
Views: 1,755
Posted By mehdik
ssDNA selection on Pippin

The way you can use the Pippin for selecting ssDNA molecules is to empirically determine at what size they come out compared to the dsDNA ladder. It works well if your process calls for the same size...
Forum: Sample Prep / Library Generation 04-10-2012, 06:50 AM
Replies: 2
Views: 3,033
Posted By mehdik
Pippin Prep conditions for miR-seq libraries

We prepare libraries according to the Illumina small RNA protocol, run them on the bioanalyzer after PCR, quantify the miR band and pool equimass on that band only. Then we use a 3% cassette and set...
Forum: Bioinformatics 12-26-2009, 01:22 PM
Replies: 7
Views: 2,774
Posted By mehdik
I installed BayesCall, I am able to install all...

I installed BayesCall, I am able to install all the prerequisites as well as BayesCall. The first part EM.py starts but I am stuck at multiple runtime erros in the python scripts. Anyone succeeded in...
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