Forum: Bioinformatics
01-20-2019, 07:33 PM
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Replies: 7
Views: 1,934
Hi Martin,
Sorry for leaving message in this...
Hi Martin,
Sorry for leaving message in this old post. But I find I meet similar situation with you and want to see if you have new idea after 2 years.
If my understanding is right, your...
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Forum: Oxford Nanopore
01-18-2018, 01:13 AM
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Replies: 4
Views: 4,360
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Forum: Sample Prep / Library Generation
01-18-2018, 12:44 AM
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Replies: 6
Views: 8,709
Hi,
I also found it! Thanks.
Actually...
Hi,
I also found it! Thanks.
Actually before reading this post, I just wondered why the second selection with less bead amount can capture small DNA. And I finally realize that it is the bead...
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Forum: Oxford Nanopore
01-18-2018, 12:36 AM
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Replies: 4
Views: 4,360
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Forum: Oxford Nanopore
01-17-2018, 06:01 PM
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Replies: 4
Views: 4,360
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Forum: Sample Prep / Library Generation
01-17-2018, 05:45 PM
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Replies: 6
Views: 8,709
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Forum: Illumina/Solexa
01-10-2018, 02:37 AM
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Replies: 1
Views: 1,313
how to self order illumina library and reagent
I am a new for Illumina, recently our lab want to prepare the illumina library by ourselves, without using the current kit. So I have the following questions would like to ask for your help.
1. Is...
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Forum: Illumina/Solexa
01-10-2018, 02:20 AM
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Replies: 14
Views: 4,430
Hi nucacidhunter,
Yes I amplify large...
Hi nucacidhunter,
Yes I amplify large targets with few long overlapping amplicons and then to assemble it, but I need to do the fragmentation to make the size suitable for illumina sequencing. So...
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Forum: Illumina/Solexa
01-09-2018, 07:26 PM
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Replies: 14
Views: 4,430
Hi Seq_learner, nucacidhunter and Vasco,
...
Hi Seq_learner, nucacidhunter and Vasco,
Thanks for all your kindness. I do understand your protocol, but one bad news is that my DNA amplicons are much longer than several hundred bp, so I need...
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Forum: Illumina/Solexa
01-08-2018, 10:20 PM
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Replies: 3
Views: 1,380
Hi luc,
I think the truseq-style barcodes...
Hi luc,
I think the truseq-style barcodes you mentioned is that kind of "multiplex index" within the adapter? The reason why we do not want to use such common way is that we want to conduct such...
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Forum: Illumina/Solexa
01-08-2018, 07:01 PM
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Replies: 3
Views: 1,380
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Forum: Illumina/Solexa
01-08-2018, 06:06 PM
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Replies: 14
Views: 4,430
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Forum: Bioinformatics
08-07-2017, 03:02 AM
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Replies: 1
Views: 1,637
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Forum: Bioinformatics
07-31-2017, 08:54 PM
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Replies: 0
Views: 1,872
Question: canu correction process
Dear Fellows,
Recently, I started to use nanopore for sequencing, and use canu for the de novo assembly. After reading the manual and conduct some test run, I am curious about canu's correction...
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