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Forum: Sample Prep / Library Generation 05-03-2012, 07:42 AM
Replies: 0
Views: 1,337
Posted By debbiehughes
TruSeq Custom Amplicon-diluting oligos?

Hi All,
I was just wondering if anyone had any experience with diluting the custom oligos (the expensive part) of Illuminas TruSeq custom amplicon?
Would be greatful of any feedback
Many thanks...
Forum: Sample Prep / Library Generation 11-21-2011, 06:06 AM
Replies: 0
Views: 1,778
Posted By debbiehughes
Agilent automated library preps-Low yields after pre-hybridisation PCR

Hiya,
I'm having problem with low yields following the pre-hybridisation PCR. I'm starting with the recomended 3ug and the fragments are the correct size but I'm only getting about 250 rather than...
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