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Forum: Illumina/Solexa 02-17-2016, 03:36 PM
Replies: 2
Views: 1,355
Posted By neurula
I haven't compared all different buffer types but...

I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.
Forum: Illumina/Solexa 01-25-2016, 03:52 PM
Replies: 33
Views: 6,785
Posted By neurula
Just to add another voice, we noticed this on our...

Just to add another voice, we noticed this on our validation runs at install in late Nov/December. Q30s dropping after 100 cycles of read 2, ending the run at ~40%. Error rates up above 7-8% as...
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