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Forum: Sample Prep / Library Generation 03-29-2017, 11:06 AM
Replies: 11
Views: 4,605
Posted By eingabe_ausgabe
http://www.nature.com/nprot/journal/v11/n8/fig_tab...

http://www.nature.com/nprot/journal/v11/n8/fig_tab/nprot.2016.090_F2.html

Does "insufficient lysis" mean cells that are only partially lysed such that only the small RNAs are released and the rest...
Forum: Sample Prep / Library Generation 03-28-2017, 11:48 AM
Replies: 11
Views: 4,605
Posted By eingabe_ausgabe
No and it may be too late for me to do that. I am...

No and it may be too late for me to do that. I am actually trying to retain the small RNAs and I don't think the presence of the ribosomes is necessarily a problem for my downstream goals. I just...
Forum: Sample Prep / Library Generation 03-27-2017, 11:46 PM
Replies: 11
Views: 4,605
Posted By eingabe_ausgabe
Interesting that I got the exact peaks / bands...

Interesting that I got the exact peaks / bands from insect fat body tissue using almost the exact same experimental set-up (zymo micro kit & Experion). I have virtually eliminated that this might be...
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