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Forum: Illumina/Solexa 11-08-2018, 03:04 AM
Replies: 2
Views: 671
Posted By NGSMicro
Thank you, most appreciated.

Thank you, most appreciated.
Forum: Illumina/Solexa 11-07-2018, 06:01 AM
Replies: 2
Views: 671
Posted By NGSMicro
What volume of adapters do you get in the nextera XT kits?

Hello,

At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)".

I want to update...
Forum: Bioinformatics 09-12-2018, 07:15 AM
Replies: 2
Views: 859
Posted By NGSMicro
I don't know for certain, but it could be that...

I don't know for certain, but it could be that trimmomatic is opening 4 of the files at a time, as it has 4 threads working.
Forum: Bioinformatics 05-08-2018, 01:32 AM
Replies: 2
Views: 398
Posted By NGSMicro
Have you tried QIIME2? It provides a few...

Have you tried QIIME2?

It provides a few higher resolution methods of clustering meaning you may be able to 'get away with' using only the forward reads.
Forum: Sample Prep / Library Generation 04-26-2018, 06:56 AM
Replies: 5
Views: 2,398
Posted By NGSMicro
Also using homemade beads with the same protocol...

Also using homemade beads with the same protocol as melop, much cheaper.

If you're worried they aren't as good you can validate them against whatever you're using now.
Forum: Illumina/Solexa 04-26-2018, 06:40 AM
Replies: 6
Views: 1,362
Posted By NGSMicro
I have been looking at ordering my own custom...

I have been looking at ordering my own custom indices - I have hypothetically made some like this:

CAAGCAGAAGACGGCATACGAGATagctcaacGTCTCGTGGGCTCGG

Where the first section binds to the flow...
Forum: Introductions 04-26-2018, 06:32 AM
Replies: 1
Views: 1,886
Posted By NGSMicro
Hello all!

Read a lot of posts here whilst trying to set up some NGS methods so thought I might as well join.

We currently have a few qPCR instruments and a MiSeq.

Currently I am looking at implementing...
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