Forum: General
08-06-2013, 11:04 AM
|
|
Replies: 0
Views: 1,626
Changing Color of Bases in BioEdit Chromatograms
Greetings!
I want to change the colors used for C and/or G in the chromatograms of BioEdit. I found code info for changing the table view. Does anyone know if we can access the code file for the...
|
|
Forum: Sample Prep / Library Generation
05-20-2013, 09:32 AM
|
|
Replies: 3
Views: 2,945
Hi Krobinson,
Thanks for your advice! I have...
Hi Krobinson,
Thanks for your advice! I have access to a Qubit for DNA quantitation, and I would like to clean the minipreps I have in hand without amplification.
It sounds like the main thing...
|
|
Forum: Sample Prep / Library Generation
05-17-2013, 01:46 PM
|
|
Replies: 3
Views: 2,945
How to prep BACs for Nextera XT DNA kit?
Greetings All,
I need help working with BACs. I need DNA from 13 BACs for use with the Nextera XT DNA kit to be sequenced on the Illumina MiSeq. I am totally totally new to working with BACs....
|
|
Forum: Illumina/Solexa
12-23-2011, 12:41 PM
|
|
Replies: 45
Views: 26,406
We tried this suggestion last week (slow ramp...
We tried this suggestion last week (slow ramp speed on the last cycle). Our bubble product was also greatly increased, but our concentrations determined by KAPA qPCR were the same as our previous...
|
|
Forum: RNA Sequencing
11-15-2011, 11:24 AM
|
|
Replies: 1
Views: 2,534
TruSeq RNA In-Line Control Sequences
Greetings,
I have recently prepared RNA libraries using the TruSeq RNA kit and the in-line controls. Does anyone have the TruSeq RNA in-line control sequences?
Thanks!
|
|
Forum: Sample Prep / Library Generation
09-27-2011, 09:42 AM
|
|
Replies: 19
Views: 8,348
|
|
Forum: Sample Prep / Library Generation
09-27-2011, 09:29 AM
|
|
Replies: 3
Views: 3,510
Hi RNAseqer,
I am guessing you mean a 1x...
Hi RNAseqer,
I am guessing you mean a 1x ratio will recover everything >~100bp. I am concerned if this is true because adapter dimers must be removed, and they are ~120bp.
Thanks,
OnUrMark
|
|
Forum: Sample Prep / Library Generation
09-26-2011, 12:35 PM
|
|
Replies: 3
Views: 3,510
TruSeq RNA initial concentration and Ampure beads
Greetings!
I am getting ready to use the TruSeq RNA kit for the first time. I noticed the concentration of Ampure beads to sample decreases as you progress through the steps to prepare an RNASeq...
|
|
Forum: Sample Prep / Library Generation
09-26-2011, 12:25 PM
|
|
Replies: 19
Views: 8,348
Hi Phillip,
Did you get to the bottom of the...
Hi Phillip,
Did you get to the bottom of the fragmentation time and median insert length issue? I spoke to a lab that uses 6 seconds, but the only person in the lab did not know what size fragment...
|
|
Forum: Sample Prep / Library Generation
06-08-2011, 11:10 AM
|
|
Replies: 3
Views: 3,157
|
|
Forum: Sample Prep / Library Generation
06-07-2011, 05:07 PM
|
|
Replies: 3
Views: 3,157
|
|
Forum: 454 Pyrosequencing
05-04-2011, 12:22 PM
|
|
Replies: 9
Views: 5,014
|
|
Forum: 454 Pyrosequencing
04-21-2011, 12:35 PM
|
|
Replies: 9
Views: 5,014
Hi Tony, would you mind sharing your gel...
Hi Tony, would you mind sharing your gel extraction technique? I believe there may be contamination from our final gel extraction that is inhibiting the sequencing reaction. The bioanalyzer shows...
|
|
Forum: Sample Prep / Library Generation
04-21-2011, 11:22 AM
|
|
Replies: 11
Views: 6,221
Hello kshankar, I too would like to know why...
Hello kshankar, I too would like to know why indexed libraries may produce lower cluster densities. How many samples per lane are you indexing?
Also, I still believe there are some contaminants...
|
|
Forum: Sample Prep / Library Generation
03-31-2011, 05:44 PM
|
|
Replies: 11
Views: 6,221
|
|
Forum: Sample Prep / Library Generation
03-29-2011, 05:24 PM
|
|
Replies: 11
Views: 6,221
|
|
Forum: Sample Prep / Library Generation
03-29-2011, 02:17 PM
|
|
Replies: 11
Views: 6,221
|
|
Forum: Sample Prep / Library Generation
03-29-2011, 01:01 PM
|
|
Replies: 11
Views: 6,221
|
|
Forum: Sample Prep / Library Generation
03-29-2011, 12:04 PM
|
|
Replies: 11
Views: 6,221
RNAseq - low cluster density - possible inhibitor?
Greetings,
We have been getting low cluster densities, and as we increase the concentration, we are getting less clusters/tile/pM. It looks like there is no way for our samples to reach the optimal...
|
|
Forum: RNA Sequencing
02-09-2011, 10:01 AM
|
|
Replies: 11
Views: 5,715
|
|
Forum: RNA Sequencing
02-09-2011, 09:49 AM
|
|
Replies: 11
Views: 5,715
|
|
Forum: RNA Sequencing
02-08-2011, 05:30 PM
|
|
Replies: 11
Views: 5,715
Thanks for your explanation and ideas. Today I...
Thanks for your explanation and ideas. Today I had several samples denatured before they were run on the bioanalyzer. The 700 bp peaks did disappear for all samples, and all samples had peaks only at...
|
|
Forum: RNA Sequencing
01-14-2011, 03:36 PM
|
|
Replies: 11
Views: 5,715
How to eliminate PE dimers from final product?
Greetings RNA-Seq'ers,
I am preparing samples for RNA-seq, and my final PCR enrichment product contains dimers and trimers of my 350bp fragments (~700bp and ~1000bp). I have gel extracted the PE...
|
|
Forum: Sample Prep / Library Generation
01-13-2011, 09:56 AM
|
|
Replies: 5
Views: 6,100
We are also seeing double peaks in our RNA-seq...
We are also seeing double peaks in our RNA-seq library preps, even though all samples have been gel extracted at 350bp. Does anyone know how it is possible to still have 700 bp fragments after gel...
|
