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Forum: Bioinformatics 12-07-2011, 11:13 AM
Replies: 10
Views: 8,371
Posted By spapillon
Make sure you adjust the standard deviation...

Make sure you adjust the standard deviation parameter accordingly when trimming out the adapters/barcodes and poor quality ends.
Forum: Bioinformatics 12-01-2011, 09:56 AM
Replies: 8
Views: 2,807
Posted By spapillon
As the error states, "Sequence must start with @:...

As the error states, "Sequence must start with @: >@ ..." Meaning that Picard is trying to read the sequence and is failing. It calls the method FastqToSam but the file you gave it is in fasta...
Forum: General 11-30-2011, 08:41 AM
Replies: 5
Views: 4,548
Posted By spapillon
Tube--, it depends on the model of you disease...

Tube--, it depends on the model of you disease and the data you have available. Are you looking at a recessive disease ? Is there consanguinity in the patients' family ? Is this a multiple patient...
Forum: Bioinformatics 11-29-2011, 08:38 PM
Replies: 0
Views: 1,858
Posted By spapillon
Problems with RNA-seq analysis results

Hi everyone,

This is my first post here, be sure to let me know if I break a rule of conduct or anything. Tricks & Tips are appreciated.

The situation:
I'm currently trying to analyze...
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