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Forum: Sample Prep / Library Generation 11-09-2011, 09:24 AM
Replies: 35
Views: 22,264
Posted By perrinwang
Hmm... that is interesting to know... also...

Hmm... that is interesting to know... also troublesome for us. I am wondering how much dNTP is retained through Ampure purification, do you have a good guess at percentage?

Here is our protocol:...
Forum: Sample Prep / Library Generation 10-27-2011, 09:02 AM
Replies: 16
Views: 17,642
Posted By perrinwang
We use first-strand buffer from SuperScript...

We use first-strand buffer from SuperScript II/III to perform the fragmention as this eliminate the need of purification before reverse-transcription.
...
Forum: Sample Prep / Library Generation 10-27-2011, 08:58 AM
Replies: 5
Views: 10,004
Posted By perrinwang
http://dx.plos.org/10.1371/journal.pone.0026426

http://dx.plos.org/10.1371/journal.pone.0026426
Forum: Bioinformatics 10-12-2010, 07:53 PM
Replies: 8
Views: 8,851
Posted By perrinwang
I am also interested in knowing a way to show...

I am also interested in knowing a way to show such average gene coverage plot... is there any software/script out there that can do this based on alignment files (SAM, BAM) and an gene annotation...
Forum: Events / Conferences 03-13-2010, 11:31 AM
Replies: 8
Views: 3,563
Posted By perrinwang
Edit: Sorry i thought i could, but the air fair...

Edit: Sorry i thought i could, but the air fair is going to bankrupt me..
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