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Search: Posts Made By: JMorlan
Forum: Bioinformatics 04-04-2014, 09:29 AM
Replies: 1
Views: 2,417
Posted By JMorlan
BEDTools coverageBed error

Recently I have been getting the following persistent error when runnng BEDTools coverageBed (ver 2.16.2 or 2.12.0):

/mnt/ngs/analysis/software/BEDTools-Version-2.16.2/bin/coverageBed: line 2:...
Forum: RNA Sequencing 01-12-2011, 12:59 PM
Replies: 0
Views: 1,901
Posted By JMorlan
Sequencing Depth and Differential Expression

I am wondering if anyone has looked at the effect of sequencing depth on the ability to detect differences in RNA-Seq count data? There are many useful references for RNA-Seq DE analysis, mostly...
Forum: Illumina/Solexa 01-12-2011, 10:23 AM
Replies: 8
Views: 3,666
Posted By JMorlan
Hi amango, I should clarify that we did not...

Hi amango,

I should clarify that we did not use our 'Homebrew' RNA-Seq protocol on the multiplexing study I mentioned in my earlier post. For multiplexing we used Illumina's now obsolete...
Forum: Illumina/Solexa 11-05-2010, 04:01 PM
Replies: 8
Views: 3,666
Posted By JMorlan
We recently completed an RNA-seq study combining...

We recently completed an RNA-seq study combining Illumina's RNA-Seq protocol and multiplex kit and running on the GAIIx. Our data quality was excellent in terms of total read yield, %Q30, % unique...
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