SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 9 of 9
Search took 0.00 seconds.
Search: Posts Made By: yuehuang
Forum: Sample Prep / Library Generation 08-24-2016, 02:41 PM
Replies: 4
Views: 1,765
Posted By yuehuang
Adam, I used to using V1-V3, couldn't get the...

Adam, I used to using V1-V3, couldn't get the exactly pure one band peak either, much more impure than the one I showed here. But I used Pippin Prep to pick the correct size libraries, which help...
Forum: Sample Prep / Library Generation 08-23-2016, 01:37 PM
Replies: 4
Views: 1,765
Posted By yuehuang
Thanks much! I'm discussing this with Brad,...

Thanks much!

I'm discussing this with Brad, your technical support. He's helpful, I believe we will figure this out.
Forum: Sample Prep / Library Generation 08-22-2016, 11:59 AM
Replies: 4
Views: 1,765
Posted By yuehuang
16S-V4 library peak at smaller bp in bioanalyzer trace

Dear All,

I used 16S-V4 kit(BIOO Scientific) to make libraries of DNA extracted from fresh plasma. But the dominant peak is at 350bp, instead of 450bp as suggested by the kit protocol. It's my...
Forum: Sample Prep / Library Generation 06-11-2015, 01:06 PM
Replies: 2
Views: 1,524
Posted By yuehuang
Thumbs up Thanks much! I will give it a shot, will keep you...

Thanks much! I will give it a shot, will keep you and this thread posted. Hopefully this try will help others.
Forum: Sample Prep / Library Generation 06-10-2015, 02:11 PM
Replies: 2
Views: 1,524
Posted By yuehuang
Nextera XT library products low average bp size

Dear All,

I'm working on some ancient blood samples(about 20yrs old), trying to do some metagenomic studies on microbiota. The issue is: most of the library prep products' average bp falling...
Forum: Illumina/Solexa 03-20-2015, 04:01 PM
Replies: 76
Views: 43,644
Posted By yuehuang
Cool thank you so much for the low-bind tube tip. I...

thank you so much for the low-bind tube tip. I tried the same procedure, got the perfect size selected and clean library prep products, but much lower yield. Lucky to me, it's still sequencable, but...
Forum: Illumina/Solexa 03-20-2015, 03:50 PM
Replies: 76
Views: 43,644
Posted By yuehuang
Did you mean, the already purified DNA library to...

Did you mean, the already purified DNA library to go another PCR at about 5 cycles, checked by bioanalyzer then go amplification again in 10-15 cycles to accumulate enough yield for sequencing?
Forum: Illumina/Solexa 03-20-2015, 10:48 AM
Replies: 76
Views: 43,644
Posted By yuehuang
Thumbs up Great job! I played with this too. But my concern...

Great job! I played with this too. But my concern focused on the size adjusted concentration, your gel is very convincible.
Forum: Illumina/Solexa 03-20-2015, 10:37 AM
Replies: 76
Views: 43,644
Posted By yuehuang
Thumbs up Thanks much for the tips. I was always bothered...

Thanks much for the tips. I was always bothered by the primer dimer when used NEB kits for library prep. The AMPure XP worked fabulously for size selection, however didn't work for getting rid of...
Showing results 1 to 9 of 9

 


All times are GMT -8. The time now is 02:18 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO