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Forum: 454 Pyrosequencing 08-04-2015, 02:40 AM
Replies: 3
Views: 6,547
Posted By flxlex
What is the total coverage (total sequenced bp...

What is the total coverage (total sequenced bp divided by genome size)? Your data should be OK for newbler in one go...
Forum: 454 Pyrosequencing 07-02-2015, 04:50 AM
Replies: 3
Views: 6,547
Posted By flxlex
Not directly out of the box. Do you need all that...

Not directly out of the box. Do you need all that data (what are you assembling)? If you do, can you do digital normalisation, or partioning (e.g., see the khmer (khmer.readthedocs.org) package)
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