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Forum: Introductions 07-25-2011, 05:36 AM
Replies: 5
Views: 9,830
Posted By herrroaa
Hi All, How can I ligate ion torrent barcodes...

Hi All,
How can I ligate ion torrent barcodes to my DNA fragments.
Regards,Tarek
Forum: Introductions 07-06-2011, 06:01 AM
Replies: 5
Views: 9,830
Posted By herrroaa
There is a long lange pcr kit offered by Qiagen....

There is a long lange pcr kit offered by Qiagen. this kit will generate a library of 8 overlapped amplicons (10 kb each) for my 70 kb gene. I think i can use it and then with biorubter or...
Forum: Introductions 07-04-2011, 12:09 AM
Replies: 5
Views: 9,830
Posted By herrroaa
I want to do this in about 50 samples and i am...

I want to do this in about 50 samples and i am interested in entire gene.
I think I can do it in one run using the 318 chip.
Forum: Introductions 06-27-2011, 05:12 AM
Replies: 5
Views: 9,830
Posted By herrroaa
ion torrent

Hi all,
I want to pre-amplify a 70Kb gene prior resequencing via ion torrnet. could any one please tell me what is the best way to do this.
Thanks
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