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Forum: Bioinformatics 06-07-2017, 11:20 AM
Replies: 347
Views: 185,376
Posted By jweger1988
Very interesting. I like the idea of quality...

Very interesting. I like the idea of quality score recalibration. If I don't have millions of reads (only between 1000-20000x avg. coverage) can I still do all of the first steps without the...
Forum: Bioinformatics 06-06-2017, 12:52 PM
Replies: 347
Views: 185,376
Posted By jweger1988
Brian, Thanks for the quick response. ...

Brian,

Thanks for the quick response.

It seems like splitting it up does give me a little better precision (as judged by a higher % of bases lost), so I'll stick with that.

On a similar...
Forum: Bioinformatics 06-06-2017, 07:23 AM
Replies: 347
Views: 185,376
Posted By jweger1988
Hi Brian, I'm trying to do some trimming of...

Hi Brian,

I'm trying to do some trimming of primer sequences from amplicons that I sequenced.

I have a viral genome that was amplified in 5 large-ish overlapping pieces (~2-3kb) and then...
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