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Forum: Ion Torrent 01-17-2012, 09:31 PM
Replies: 28
Views: 18,139
Posted By singaporeseq
Has anyone seen any public/customer data on the...

Has anyone seen any public/customer data on the 318 chip?
Forum: Ion Torrent 01-10-2012, 10:15 PM
Replies: 88
Views: 153,768
Posted By singaporeseq
So I guess it's the end of the scalability claim...

So I guess it's the end of the scalability claim for PGM...unless you want to pay 200K for a Ion Proton....I don't imagine we'll be seeing a 320 chip ...
Forum: Ion Torrent 01-03-2012, 10:56 PM
Replies: 28
Views: 18,139
Posted By singaporeseq
I see, just closer together now...more crosstalk...

I see, just closer together now...more crosstalk with closer wells then?
Forum: Ion Torrent 01-03-2012, 08:50 PM
Replies: 28
Views: 18,139
Posted By singaporeseq
Thanks for the picture! With smaller wells are we...

Thanks for the picture! With smaller wells are we also expecting to see a drop in signal? Wondering what the delay for the 318 is...was originally supposed to be released in Mid 2011...
Forum: Ion Torrent 12-15-2011, 04:29 PM
Replies: 7
Views: 4,341
Posted By singaporeseq
Have you actually tried the MiSeq? I have and it...

Have you actually tried the MiSeq? I have and it is that easy
Forum: Ion Torrent 12-12-2011, 11:30 PM
Replies: 15
Views: 25,939
Posted By singaporeseq
Why are you selling?

Why are you selling?
Forum: Ion Torrent 12-12-2011, 11:26 PM
Replies: 7
Views: 4,341
Posted By singaporeseq
The Miseq workflow is so easy. Can do Nextera...

The Miseq workflow is so easy. Can do Nextera sample prep in under 2hrs, quant your sample and load in the reagent cartridge and literally press go. All bridge amp, seq an d data analysis done...
Forum: Ion Torrent 11-28-2011, 07:38 AM
Replies: 3
Views: 6,159
Posted By singaporeseq
Thanks for the info. I was interested in both...

Thanks for the info. I was interested in both amplicons and also bacterial whole genome.
I wanted to see if anyone has experience with the ion barcode kits. I can't find much information on them.
Forum: Ion Torrent 11-28-2011, 01:00 AM
Replies: 3
Views: 6,159
Posted By singaporeseq
Multiplexing with ion torrent?

I am looking for a protocol or personal experience in doing multiplexing with ion torrent. Has anybody done this?
The app note is not very informative
Forum: Ion Torrent 11-28-2011, 12:33 AM
Replies: 15
Views: 7,349
Posted By singaporeseq
Experience with multiplexing on ion torrent?

Has anyone seen any protocols for multiplexing on ion torrent? I can't find a protocol anywhere. Has anybody done this/ used the barcode kit?
Forum: Sample Prep / Library Generation 09-12-2011, 07:32 AM
Replies: 4
Views: 2,739
Posted By singaporeseq
Yes, planning on using Covaris. Thanks so much...

Yes, planning on using Covaris. Thanks so much for your help!
Forum: Sample Prep / Library Generation 09-09-2011, 08:27 PM
Replies: 4
Views: 2,739
Posted By singaporeseq
Sonication of non gDNA

Hello, I have a basic question. I am extracting samples that will be around 3.5 Mb and only done sonication on whole genome. Can I still use it with the smaller input or will I over fragment? Is...
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