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Forum: Illumina/Solexa 07-26-2018, 12:25 AM
Replies: 14
Views: 778
Posted By SarahAurora
Unluckily, we don't have a bioanalyzer. We were...

Unluckily, we don't have a bioanalyzer. We were using the MultiNA device but on it, we saw more or less correct bands (anyways, nothing outside the expected length range). Yes right, reads running...
Forum: Illumina/Solexa 07-25-2018, 04:53 AM
Replies: 14
Views: 778
Posted By SarahAurora
600 cycles. This explains the bad quality of the...

600 cycles. This explains the bad quality of the read-ends but it doesn't explain why in some runs, the quality is better and the reads are longer than in the last runs (Run 7 and 10)
Forum: Illumina/Solexa 07-25-2018, 04:35 AM
Replies: 14
Views: 778
Posted By SarahAurora
Run 5: 12pM input - was ok (Q30 58%) but not so...

Run 5: 12pM input - was ok (Q30 58%) but not so many reads as expected.
Run 7: 10pM input - bad quality and less PF and effective reads
Run 9: 10pM input - overclustered, no results due to too...
Forum: Illumina/Solexa 07-25-2018, 03:26 AM
Replies: 14
Views: 778
Posted By SarahAurora
15% phiX. We also mix the libraries with...

15% phiX. We also mix the libraries with amplicons of other (not STR amplicons) genetic loci
Forum: Illumina/Solexa 07-25-2018, 03:20 AM
Replies: 14
Views: 778
Posted By SarahAurora
They are not finished yet. I have fastcq reports...

They are not finished yet. I have fastcq reports only for single samples, not for the whole run. But the last 3 runs showed smaller reads than before
Forum: Illumina/Solexa 07-25-2018, 01:47 AM
Replies: 14
Views: 778
Posted By SarahAurora
MiSeq amplicon sequencing of STRs: Low PF and effective reads due to short reads

Dear all,

we are currently working on the developement and validation of a MiSeq-based in-house developed NGS assay, using amplicons targeting forensic STR loci. We are using paired-end sequencing...
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