Forum: Illumina/Solexa
03-07-2019, 03:54 PM
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Replies: 1
Views: 1,581
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Forum: Illumina/Solexa
03-07-2019, 10:31 AM
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Replies: 3
Views: 919
Hi! thanks for your reply. Actually I asked the...
Hi! thanks for your reply. Actually I asked the reason why they didinīt performed a demultiplex propperly and they explained me that they used a protocol to improve quality and supposedly bcl2fastq...
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Forum: Illumina/Solexa
03-06-2019, 12:27 PM
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Replies: 3
Views: 919
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Forum: Bioinformatics
10-09-2012, 07:09 AM
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Replies: 4
Views: 3,562
Hi Jeremy, thanks for your answer...
Yes I...
Hi Jeremy, thanks for your answer...
Yes I tried to use this manual, but when I was readind I didin't notice a real help. I tried to use the configuration example that appears in this site, and I...
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Forum: Bioinformatics
10-08-2012, 11:35 AM
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Replies: 4
Views: 3,562
SOAPdenovo-trans without paired reads
Hi all of you,
I'm trying to configure a transcriptome sequence assembly using SOAPdenovo-trans, but I can't find a appropiate manual, my problem starts because I don't have a paired-reads...
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Forum: Bioinformatics
09-14-2012, 10:35 AM
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Replies: 3
Views: 4,414
KO (Kegg orthology) database to download
Hi all of you, my apologies if I answer you too much times, but I make questions of a parter too.
Well my problem is that we are trying to download KO database to align it with a reads set but we...
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Forum: Bioinformatics
09-14-2012, 06:19 AM
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Replies: 2
Views: 4,625
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Forum: Bioinformatics
09-13-2012, 12:39 PM
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Replies: 2
Views: 4,625
SRA format file. How to use it?
I downloaded a sequencing proyect from SRA (http://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi) and I downloaded a ile which contains a lot of information of raw reads. My problem starts when I try to...
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Forum: Bioinformatics
08-31-2012, 12:12 PM
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Replies: 1
Views: 1,084
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Forum: Bioinformatics
08-20-2012, 02:20 PM
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Replies: 3
Views: 1,688
Degenerate primers desing software
Hi all of you,
I'm looking for a software to design degenerate primers to make a multiplex PCR to try to close a little genome who has 10 contigs. I tried to use some primers developers softwares...
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Forum: Bioinformatics
08-16-2012, 01:05 PM
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Replies: 2
Views: 1,088
where can I find a good EST anotation pipeline?
Hi, i'm trying to find a software preferently a local installation to make an EST annotation, but I can't find a dependable software. Where can I find something like this? It's for a transcriptome. ...
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Forum: Bioinformatics
08-01-2012, 08:16 AM
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Replies: 1
Views: 1,417
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Forum: Bioinformatics
07-12-2012, 01:37 PM
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Replies: 5
Views: 4,868
its name is bp_aacomp.pl, this script can count...
its name is bp_aacomp.pl, this script can count the aminoacids of a FASTA format file. This doesn't work good with multifasta file, because it takes count of all the aminoacids of the file. If you...
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Forum: Bioinformatics
07-10-2012, 07:58 AM
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Replies: 3
Views: 1,904
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Forum: Bioinformatics
07-06-2012, 06:53 AM
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Replies: 3
Views: 1,904
Blast problem in metagenomics alignment
Hi there, well I presented an annoying problem with blast. The problem is the next:
We are triying to aling a metagenomic sequence project with local blast, using software packages included in...
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Forum: 454 Pyrosequencing
06-13-2012, 02:11 PM
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Replies: 4
Views: 1,888
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Forum: 454 Pyrosequencing
06-04-2012, 11:48 AM
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Replies: 2
Views: 1,781
Hi, your first question is related with the...
Hi, your first question is related with the quality standard used in the sequencing, 10 or lower as you told, explains a bad quality read (usually that this number can be 64) , but you have to...
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Forum: 454 Pyrosequencing
06-01-2012, 12:25 PM
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Replies: 4
Views: 1,888
I was searching for a closely related reference...
I was searching for a closely related reference but I didin't find anything. And we don't have a paired end sequence data, for this I am designing primers, but as I was not as clear of the strategy...
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Forum: 454 Pyrosequencing
06-01-2012, 08:36 AM
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Replies: 6
Views: 2,284
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Forum: 454 Pyrosequencing
06-01-2012, 08:30 AM
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Replies: 4
Views: 1,888
problems closing gaps phage genome
Hi there!,
I suscribe to this forum cause I notice that is really useful for everyone who is working un NGS technology.
Well, my trouble starts with de gap closing of a phage... there are...
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