Forum: Oxford Nanopore
09-23-2016, 06:28 AM
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Replies: 2
Views: 5,675
minIon for plasmid sequencing
I was just wondering if anyone has used the minIon for plasmid sequencing? I suppose it might be overkill and a waste or reagents for such a small task. But I am asking because I have large...
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Forum: Bioinformatics
08-22-2013, 09:17 AM
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Replies: 9
Views: 9,931
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Forum: Bioinformatics
07-26-2013, 02:41 PM
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Replies: 24
Views: 8,069
I would suggest carefully reading the manual...
I would suggest carefully reading the manual here:
http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic/TrimmomaticManual_V0.30.pdf
There are some powerful options like using...
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Forum: Bioinformatics
07-26-2013, 02:37 PM
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Replies: 5
Views: 4,108
BWA MEM question
I am using BWA MEM to map 250 bp MiSeq PE reads. I am doing targeted genomic sequencing, enriching the library for specific transgenic sequences. I make a reference sequence, map all of the reads...
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Forum: Bioinformatics
07-26-2013, 02:26 PM
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Replies: 24
Views: 8,069
I second the vote for timmomatic. Once you get...
I second the vote for timmomatic. Once you get the hang of how to make the adapter seqeunce file, it is really easy to use, fast and takes care of all of the paired end info stuff. A very powerful...
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Forum: Bioinformatics
05-16-2013, 03:45 PM
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Replies: 10
Views: 1,637
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Forum: Bioinformatics
05-10-2013, 03:59 AM
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Replies: 3
Views: 1,094
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Forum: Bioinformatics
05-09-2013, 04:01 PM
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Replies: 3
Views: 1,094
Finding a read's mate
I have a BWA alignment of reads to a small (50 kb) reference sequence. It is a transgenic sequence inserted into a host cell genome. I want to be able to locate the insert's position in the host...
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