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Forum: Illumina/Solexa 03-29-2016, 10:22 PM
Replies: 0
Views: 839
Posted By Catherine_Burke
MuSeek library prep kit for Illumina

Has anyone used the MuSeek library prep kit for Illumina libraries? I noticed in the Ion Torrent MuSeek kit, they give you the adaptor and primer sequences, but not in the Illumina compatible kit.
...
Forum: Illumina/Solexa 11-13-2014, 01:36 PM
Replies: 55
Views: 30,338
Posted By Catherine_Burke
Has anyone used the Caporaso protocol with the V3...

Has anyone used the Caporaso protocol with the V3 600 cycles kits? We have previously used the V2 500 cycles kits successfully, but when we tried it with the V3 600 cycle kit, we got really low...
Forum: Metagenomics 03-17-2014, 04:10 PM
Replies: 9
Views: 6,409
Posted By Catherine_Burke
"is there anything suggesting that hi-fidelity...

"is there anything suggesting that hi-fidelity polymerases are more prone to chimera-formation/dropping extension runs?"

The link given above (here again...
Forum: Metagenomics 08-14-2013, 02:34 PM
Replies: 9
Views: 6,409
Posted By Catherine_Burke
"Above this template concentration, they see ~50%...

"Above this template concentration, they see ~50% chimera formation with 39 cycles (they didn't test less than this). "

Sorry, that should have read 30 cycles...
Forum: Metagenomics 08-14-2013, 02:33 PM
Replies: 9
Views: 6,409
Posted By Catherine_Burke
Thanks for all your replies. I did see the...

Thanks for all your replies.

I did see the Lahr and Katz (2009) paper, which is what got me worried about phusion! They do show very little recombination with small amounts of starting template,...
Forum: Metagenomics 08-13-2013, 11:10 PM
Replies: 9
Views: 6,409
Posted By Catherine_Burke
Polymerase of choice for generating 16S amplicons?

I'm currently generating 16S amplicon libraries for Illumin sequencing using Phusion polymerase, but we have noticed a high level of recombination during the enrichment PCR (~30% of sequences have...
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