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Forum: Illumina/Solexa 05-18-2010, 05:35 AM
Replies: 145
Views: 289,620
Posted By mx1970
Hi, I want to do directional mRNA seq. I have...

Hi,
I want to do directional mRNA seq. I have to use the Small RNA Sequencing Primer during the primer hybridization.
Does anybody know at which concentration should I adjust this primer?
thanks
Forum: Illumina/Solexa 05-18-2010, 05:12 AM
Replies: 28
Views: 10,948
Posted By mx1970
RNA Sequencing primer

Does anybody know at which concentration is necessary adjust the small RNA sequencing primer to perform the primer hybridization for the directinal mRNA sequencing protocol?

Thanks
Forum: Sample Prep / Library Generation 10-14-2009, 03:43 AM
Replies: 4
Views: 1,805
Posted By mx1970
Is possible to stain with SYBR green

We are going to buy a transilluminator to cut the slide from the gel to make the library and We are thinking in get a transiluminator of blue light instead UV.

Could be possible to stain the DNA...
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