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Search: Posts Made By: dannyhi321
Forum: Illumina/Solexa 04-08-2016, 08:09 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
I think you will find your logic is unsound, a...

I think you will find your logic is unsound, a 2x300 kit that only "works" at 2x275 "sometimes" is not quality!

Do you think you could share the Q Score graphs? would be interesting!
Forum: Illumina/Solexa 04-04-2016, 11:26 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
With all due respect, I don't regard the...

With all due respect, I don't regard the statement "There will be no public announcement until we have 100% identified the problem and removed it" relating to a known problem with a core reagent as...
Forum: Illumina/Solexa 04-04-2016, 07:56 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
Do you think no feedback re the reagent issue is...

Do you think no feedback re the reagent issue is acceptable?
Forum: Illumina/Solexa 04-04-2016, 07:36 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
There IS feedback from illumina: At...

There IS feedback from illumina:

At Illumina's UK UGM the team discussed the low quality at the end of long-read MiSeq kits. They acknowleged there was an issue and that they were actively working...
Forum: Illumina/Solexa 04-04-2016, 05:05 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
Exclamation Is anyone currently using these v3 600 kits?...

Is anyone currently using these v3 600 kits? Anymore feedback from Illumina?
Forum: Illumina/Solexa 04-02-2016, 03:47 AM
Replies: 68
Views: 17,203
Posted By dannyhi321
Has anyone heard of any developments re this...

Has anyone heard of any developments re this issue?
Forum: Illumina/Solexa 04-01-2016, 06:43 AM
Replies: 17
Views: 6,747
Posted By dannyhi321
As long as they are not LESS than a year of...

As long as they are not LESS than a year of course (V3 600)!
Forum: Illumina/Solexa 03-18-2016, 03:34 AM
Replies: 10
Views: 1,403
Posted By dannyhi321
Hi, Interesting thread. I have seen...

Hi,

Interesting thread.

I have seen this with dust or water droplets on the flow cell surface - do you have a "leaky" flow cell connection for example - on the Miseq system itself?

I have...
Forum: Illumina/Solexa 03-18-2016, 03:27 AM
Replies: 10
Views: 1,403
Posted By dannyhi321
Hi! Do you have the forth thumbnail image -...

Hi!
Do you have the forth thumbnail image - would be very interesting ?
Forum: Illumina/Solexa 03-08-2016, 03:19 AM
Replies: 2
Views: 946
Posted By dannyhi321
Thanks for that - why didn't they just fix the...

Thanks for that - why didn't they just fix the leak?
Forum: Illumina/Solexa 03-08-2016, 03:12 AM
Replies: 10
Views: 1,349
Posted By dannyhi321
Looks like a focusing/imaging problem - did you...

Looks like a focusing/imaging problem - did you get any usable data?
Forum: Illumina/Solexa 03-07-2016, 02:07 AM
Replies: 2
Views: 946
Posted By dannyhi321
Exclamation Miseq Flow cell leakage Problems

I have been having problems with leakage from one of my Miseq flow cells. There is pitting on the heater/slide holder, from salt/fluid build up it seems and resulting erosion - I get contradictory...
Forum: General 03-06-2016, 07:33 AM
Replies: 12
Views: 1,272
Posted By dannyhi321
Smile Pure academics/students, researchers should not...

Pure academics/students, researchers should not be discouraged (or placed under any pressure- as your communication could be construed to imply) from contacting me - it is my mission, and my...
Forum: General 03-02-2016, 10:03 AM
Replies: 12
Views: 1,272
Posted By dannyhi321
consent: kənˈsɛnt/Submit noun 1. ...

consent:
kənˈsɛnt/Submit
noun
1.
permission for something to happen or agreement to do something.
"no change may be made without the consent of all the partners"
synonyms: agreement, assent,...
Forum: The Pipeline 03-02-2016, 09:46 AM
Replies: 14
Views: 4,390
Posted By dannyhi321
Do you have a ref for that? I would find it...

Do you have a ref for that? I would find it useful and interesting, as I am sure, many others will.
Forum: The Pipeline 03-02-2016, 09:10 AM
Replies: 14
Views: 4,390
Posted By dannyhi321
Exclamation In the light of the following information, yes I...

In the light of the following information, yes I would say so, someone should inform Illumina, if they are not already aware.:-

Domain Name: genedrabio.com
Registry Domain ID:...
Forum: The Pipeline 02-29-2016, 11:36 AM
Replies: 14
Views: 4,390
Posted By dannyhi321
Suspicious ..yes.. Plagiarism, hypocrisy AND...

Suspicious ..yes..

Plagiarism, hypocrisy AND racism…classy!
Forum: General 02-29-2016, 09:33 AM
Replies: 12
Views: 1,272
Posted By dannyhi321
Really ?:- that doesn’t sound very scientific to...

Really ?:- that doesn’t sound very scientific to me. Not to mention immoral/illegal ! - or I might just not understand “digital” data!

You cannot obtain knowledge without integrity, all...
Forum: General 02-27-2016, 03:57 AM
Replies: 12
Views: 1,272
Posted By dannyhi321
But what about the consent of the sequencing...

But what about the consent of the sequencing center? - just as with the music example (more to come on that - strange coincidence !) - the resources where used with the owners consent - not so with...
Forum: General 02-27-2016, 01:10 AM
Replies: 12
Views: 1,272
Posted By dannyhi321
Cool Knowledge of the law as it applies to data rights

Dear Seq users,

I am interested in peoples knowledge of the law in terms of rights to sequencing data generated on their/someone else's system(s). The question is this, if one party generates...
Forum: General 02-26-2016, 05:32 AM
Replies: 7
Views: 1,287
Posted By dannyhi321
I thought discrimination was not tolerated on...

I thought discrimination was not tolerated on this site!?
Forum: General 02-26-2016, 01:17 AM
Replies: 7
Views: 1,287
Posted By dannyhi321
That's blatantly not true, take the recent IBS V...

That's blatantly not true, take the recent IBS V Illumina case as an example.

Ever heard of David V Goliath?
Forum: Illumina/Solexa 02-22-2016, 11:49 AM
Replies: 49
Views: 8,165
Posted By dannyhi321
Exclamation Yes - it was not JUST Illumina - but Illumina can...

Yes - it was not JUST Illumina - but Illumina can help sort the situation out.

So now Illumina have "lost the keys", so to speak, to my Miseq data and just because I gave someone the benefit of...
Forum: Illumina/Solexa 02-22-2016, 04:18 AM
Replies: 49
Views: 8,165
Posted By dannyhi321
Exclamation UPDATE:- So Illumina have resorted to legal...

UPDATE:-

So Illumina have resorted to legal threats (more on this later) and have completely ignored my requested to relinquish access to 2 years worth of my Miseq data stored on Basespace - not...
Forum: Illumina/Solexa 02-20-2016, 11:10 AM
Replies: 7
Views: 1,607
Posted By dannyhi321
you might be interested in tis thread.. not that...

you might be interested in tis thread.. not that its third party - i own the machines from new.. but a picture emerges ...

http://seqanswers.com/forums/showthread.php?t=44849&highlight=party
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