Forum: Core Facilities
09-20-2013, 12:41 PM
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Replies: 11
Views: 4,153
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Forum: Core Facilities
09-18-2013, 05:29 PM
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Replies: 11
Views: 4,153
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Forum: Core Facilities
09-17-2013, 11:27 AM
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Replies: 11
Views: 4,153
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Forum: Core Facilities
09-17-2013, 08:20 AM
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Replies: 11
Views: 4,153
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Forum: Core Facilities
09-17-2013, 06:40 AM
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Replies: 11
Views: 4,153
Metagenomics/Metatrancriptomics Sequencing
Hello Everyone,
We have some ice samples and want to know where could we get it sequenced. We want to do metagenomics and metatranscriptomics.
Any kind of information will be highly appreciated.
...
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Forum: Metagenomics
09-13-2013, 11:51 AM
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Replies: 0
Views: 1,672
Metagenomics/Metatrancriptomics Sequencing
Hello Everyone,
We have some ice samples and want to know where could we get it sequenced. We want to do metagenomics and metatranscriptomics.
Any kind of information will be highly...
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Forum: Bioinformatics
05-20-2013, 08:52 AM
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Replies: 4
Views: 1,456
Thanks ppgardne,
I have reads from...
Thanks ppgardne,
I have reads from sequencing and I want to align these to ribosomal database based on secondary structure. I have aligned based on sequence and want to see if aligning based on...
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Forum: Bioinformatics
05-17-2013, 11:47 AM
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Replies: 4
Views: 1,456
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Forum: Bioinformatics
05-15-2013, 01:45 PM
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Replies: 4
Views: 1,456
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Forum: Metagenomics
04-30-2013, 09:25 AM
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Replies: 5
Views: 2,011
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Forum: Metagenomics
04-29-2013, 03:21 PM
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Replies: 5
Views: 2,011
Thanks JackieBadger,
I did some 16S rDNA...
Thanks JackieBadger,
I did some 16S rDNA sequencing from the samples collected from ice and they were preserved in ethanol. But, now I need to do metatranscriptomic for ice samples and I am...
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Forum: Metagenomics
04-29-2013, 01:34 PM
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Replies: 5
Views: 2,011
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Forum: Bioinformatics
02-12-2013, 02:09 PM
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Replies: 9
Views: 2,409
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Forum: Bioinformatics
02-12-2013, 08:01 AM
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Replies: 9
Views: 2,409
Thanks GenoMax,
I did get the original file...
Thanks GenoMax,
I did get the original file from the facility but as I was new to the field I asked them to demultiplex it for me and got the file I showed above. So, now I have both the files...
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Forum: Bioinformatics
02-11-2013, 06:27 PM
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Replies: 9
Views: 2,409
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Forum: Bioinformatics
02-11-2013, 01:13 PM
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Replies: 9
Views: 2,409
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Forum: Bioinformatics
02-11-2013, 12:51 PM
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Replies: 9
Views: 2,409
submitting data to SRA
Hi,
I am trying to submit a 16s rRNA reads from Illumina on SRA. I have reached to level where it is asking me the following things:
Flowcell, Lane, Filename, md5checksum.
I have the...
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Forum: Metagenomics
01-11-2013, 11:10 AM
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Replies: 3
Views: 2,546
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Forum: Metagenomics
01-11-2013, 09:08 AM
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Replies: 3
Views: 2,546
V4 region primers
Hi Everyone,
I may be asking very basic question but I want to know what does 'H', 'V' and 'W' indicate in the reverse primer of V4 region of 16s rRNA. The primer sequence is...
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Forum: Bioinformatics
10-25-2012, 09:09 AM
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Replies: 2
Views: 1,224
aligned and unaligned database
Hi Everyone,
I am very confused here, I am not able to get the difference between aligned and unaligned database.
Here is an example of an aligned database:
>AF515816.1...
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Forum: Illumina/Solexa
10-17-2012, 02:44 PM
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Replies: 13
Views: 11,656
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Forum: Illumina/Solexa
10-17-2012, 02:42 PM
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Replies: 13
Views: 11,656
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Forum: Illumina/Solexa
10-17-2012, 02:40 PM
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Replies: 13
Views: 11,656
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Forum: Illumina/Solexa
10-17-2012, 11:47 AM
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Replies: 13
Views: 11,656
Demultiplex Illumina reads
Hi Everyone,
I am kind of stuck with my Illumina data, I want to remove the barcodes from my reads. My read file looks like this
@HWI-ST1035:115:C0RG7ACXX:5:1101:1216:2040 1:N:0:...
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Forum: Bioinformatics
10-04-2012, 01:10 PM
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Replies: 6
Views: 1,711
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