Forum: Sample Prep / Library Generation
05-02-2017, 06:50 AM
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Replies: 2
Views: 2,316
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Forum: Sample Prep / Library Generation
02-23-2017, 04:26 AM
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Replies: 2
Views: 3,449
Hi,
did you get any feedback on those...
Hi,
did you get any feedback on those libraries?
I have got something similar but from different cells.
Did you sequenced them at the end? if yes, how was the result?
Thanks
Dam
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Forum: Bioinformatics
02-23-2015, 06:17 AM
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Replies: 7
Views: 4,911
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Forum: Bioinformatics
11-25-2014, 03:43 PM
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Replies: 1
Views: 2,082
Question for experts in normalization
Hi Guys
I have replicated RNA-seq samples from 6 populations at 2 different treatments (benign and heat stress) repeated at two separate times (year 1 and year 2).
I am looking for the best way...
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Forum: Bioinformatics
08-04-2014, 06:23 AM
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Replies: 7
Views: 4,911
Tanks for the reply colindaven,
(forgot to...
Tanks for the reply colindaven,
(forgot to mention) unfortunately there is no reference genome... therefore I need to get the transcriptome annotated as accurate as possible for the pop gen...
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Forum: Bioinformatics
08-04-2014, 05:50 AM
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Replies: 7
Views: 4,911
Big question - Transcriptome annotation
Hi guys,
I have just finished to assemble my transcriptome ( I used Trinity, N50 is 2000, I am quite happy with it).
Now I would like to annotate it, particularly for doing some SNPs calling and...
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Forum: Bioinformatics
01-14-2014, 02:34 AM
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Replies: 2
Views: 1,547
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Forum: Bioinformatics
01-13-2014, 02:52 AM
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Replies: 2
Views: 1,547
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Forum: Bioinformatics
09-24-2013, 09:03 AM
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Replies: 15
Views: 9,773
Thank you guys,
I did actually decide to try...
Thank you guys,
I did actually decide to try both approaches :)
My result will follow in this thread.
@westerman, also, what tool will allow me to combine data sets using protein translations?...
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Forum: Bioinformatics
09-09-2013, 01:14 AM
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Replies: 15
Views: 9,773
Hi Jeremy
Unfortunately there is not a...
Hi Jeremy
Unfortunately there is not a sequenced genome for this species yet
The closest sequenced genome is D. pseudoobscura, which is already 9% divergent (just at coding sequences).
Thanks....
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Forum: Bioinformatics
09-06-2013, 06:51 AM
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Replies: 15
Views: 9,773
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Forum: Bioinformatics
09-05-2013, 08:06 AM
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Replies: 15
Views: 9,773
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Forum: Sample Prep / Library Generation
04-12-2013, 02:03 AM
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Replies: 0
Views: 1,746
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Forum: Illumina/Solexa
03-01-2013, 09:42 AM
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Replies: 1
Views: 1,547
BGI illumina RNA-seq comparison
Hi there
I want to ask if someone compared data from BGI Illumina 50bp single end RNA-seq, where they use an optimized Illumina protocol for libraries preparation, with same kind of data (samples)...
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