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Forum: Pacific Biosciences 10-13-2016, 04:12 PM
Replies: 9
Views: 2,086
Posted By ribodoc
Thank you for the response.

Thank you for the response.
Forum: Pacific Biosciences 10-13-2016, 01:40 PM
Replies: 9
Views: 2,086
Posted By ribodoc
Occasionally we see this occur and it was time to...

Occasionally we see this occur and it was time to put it out to the community. We typically do a titration of SMRTbell concentration for loading and then do a production run on a strip of SMRTcells....
Forum: Pacific Biosciences 10-13-2016, 01:16 PM
Replies: 9
Views: 2,086
Posted By ribodoc
Within a week, with the same SMRTbell template...

Within a week, with the same SMRTbell template and loading concentration went from 47.44% P1 to 26.64% P1. Accuracy dipped from 0.841 to 0.831. Polymerase read length N50 had a smaller drop from...
Forum: Pacific Biosciences 10-13-2016, 07:53 AM
Replies: 9
Views: 2,086
Posted By ribodoc
PacBio RS II SMRTcell performance variability

We are observing increased variability in the performance of SMRTcells by lot, strip and within a strip. Using the same SMRTbell template on different sequencing runs the variability can be as much...
Forum: Sample Prep / Library Generation 05-07-2013, 10:47 AM
Replies: 3
Views: 1,708
Posted By ribodoc
Thanks for the reply. We've been using the...

Thanks for the reply. We've been using the metabacteria kit for awhile with good results so our recent experience is an outlier however our level of confidence in the product has decreased. Beyond...
Forum: Sample Prep / Library Generation 05-06-2013, 03:58 PM
Replies: 3
Views: 1,708
Posted By ribodoc
We recently used ribo-zero for metabacteria and...

We recently used ribo-zero for metabacteria and have >50% of our reads mapping to bacillus subtilis rRNA and e. coli rRNA. It appears that the capture failed for some reason. Does anyone perform QC...
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