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Forum: Bioinformatics 05-27-2014, 02:16 PM
Replies: 0
Views: 1,674
Posted By jvelotta
Trinity's insilico_read_normalization.pl utility

Hello,

I have two questions about this utility:

1) Is there a standard, perhaps conservative, choice for the value of --max_cov?

2) Is there a way to determine whether --KMER_SIZE and...
Forum: RNA Sequencing 05-07-2013, 01:15 PM
Replies: 0
Views: 2,223
Posted By jvelotta
RNA Integrity on Agilent's Bioanalyzer

Hello,

I am running RNA on the Agilent Bioanalyzer (RNA nano chip) and am getting an error with some samples, which otherwise appear normal (at least, the gels and electropherograms look normal). ...
Forum: Bioinformatics 04-09-2013, 11:37 AM
Replies: 4
Views: 1,498
Posted By jvelotta
Thanks. Any experience with either of these...

Thanks. Any experience with either of these packages? Are there major differences?
Forum: Bioinformatics 04-09-2013, 06:39 AM
Replies: 4
Views: 1,498
Posted By jvelotta
RNA Seq: differential gene expression with multiple factors

Hello,

I'm looking to assess differential gene expression using Illumina HiSeq reads. I have a two-factor experimental design (three divergent fish populations each treated at two salinities). ...
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