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Forum: Illumina/Solexa 12-03-2013, 03:23 PM
Replies: 20
Views: 16,269
Posted By BIG_SNP


Many of the combinations you have listed require index 17:

for example...

A and B indexes:
7 17
3 17 20
4 17 24
Forum: Illumina/Solexa 11-17-2011, 04:04 PM
Replies: 38
Views: 19,988
Posted By BIG_SNP
We have had great success using the NuGen library...

We have had great success using the NuGen library prep. Their adapters have inline barcodes which adds to the diversity for the first cycles and allows sequences to pass filter. After passing...
Forum: Illumina/Solexa 10-27-2011, 05:13 PM
Replies: 22
Views: 9,750
Posted By BIG_SNP
We often go as low as 100ng input for DNA...

We often go as low as 100ng input for DNA libraries. Alternatively as others have mentioned we have also had good luck with 1ug input and dramatically reducing the number of PCR cycles.
Forum: Illumina/Solexa 10-27-2011, 05:10 PM
Replies: 20
Views: 16,269
Posted By BIG_SNP
We have had good success running low (or no)...

We have had good success running low (or no) diversity samples on the HiSeq using the Nugen kit which uses in-line barcodes. We simply use multiple inline barcodes for each sample which tricks the...
Forum: Illumina/Solexa 09-21-2011, 01:56 PM
Replies: 16
Views: 11,734
Posted By BIG_SNP

If you believe the problem is with the Illumina adapters or kits themselves perhaps you should try the library prep kits from NuGEN. We have been using them with good success for the past 6 months. ...
Forum: Illumina/Solexa 09-21-2011, 01:34 PM
Replies: 75
Views: 33,870
Posted By BIG_SNP

We use the KAPA Biosciences kit. It comes with standards and everything you need to quant your adapted libs. We use this routinely with the bioanalyzer to QC our libraries before sequencing.
Forum: Service Providers 12-22-2010, 03:39 PM
Replies: 11
Views: 5,136
Posted By BIG_SNP

You can try EA on the east coast since you are in GA
Forum: Illumina/Solexa 12-22-2010, 03:37 PM
Replies: 3
Views: 2,494
Posted By BIG_SNP
BAD HiSeq flowcells

What percent of your HiSeq flowcells have been 'bad'; ie have the wavy lines of clusters? Recently we have been experiencing >50% of our flowcells with this problem and are quite frustrated and...
Forum: Illumina/Solexa 12-22-2010, 03:28 PM
Replies: 35
Views: 50,342
Posted By BIG_SNP
TruSeq adapter sequences?

Does anyone know the sequences for the new TruSeq adapters?

Forum: Illumina/Solexa 03-08-2010, 10:29 AM
Replies: 32
Views: 24,060
Posted By BIG_SNP
SPRI lib prep?

Does anyone have a protocol for using SPRI beads for the Illumina lib prep? Our lab would like to omit running gels and gel purification steps. I am curious how to utilize the SPRI beads for size...
Forum: Illumina/Solexa 03-03-2010, 10:58 AM
Replies: 20
Views: 6,196
Posted By BIG_SNP
Angry I am so happy I found this post. We are having...

I am so happy I found this post. We are having the exact same problems; runs pausing due to lack of space on the D: drive. Tech support has blamed our network multiple times over the phone and...
Forum: Illumina/Solexa 02-03-2010, 09:35 AM
Replies: 9
Views: 5,152
Posted By BIG_SNP
Lib prep automation?

Has anyone automated any or all of the Illumina library prep steps on a robot? Beckman, Tecan, Hamilton? Any help will be appreciated. Thanks
Forum: Illumina/Solexa 08-20-2009, 03:39 PM
Replies: 0
Views: 1,451
Posted By BIG_SNP
GAIIx cooler condensation

Is anyone running the new IIx upgrade experiencing condensation problems with the new reagent cooler? The new cooler box that holds the 175mL conical reagent tubes keeps collecting so much...
Forum: Illumina/Solexa 08-20-2009, 03:34 PM
Replies: 0
Views: 2,674
Posted By BIG_SNP
NEB lib prep kit

Hello All

Just wanted to let people know that we have tested and had good results using the NEB lib prep kit on our GAIIx. The kit has everything you will need except the oligos and is a much...
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