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Forum: Illumina/Solexa 02-26-2020, 01:20 PM
Replies: 3
Views: 1,248
Posted By mdegraaf
Thank you for your answer. I did contact the...

Thank you for your answer. I did contact the provider but haven't heard back from them yet.
Forum: Sample Prep / Library Generation 02-25-2020, 01:46 PM
Replies: 7
Views: 3,331
Posted By mdegraaf
Hi, I apologize for the late reply, I was very...

Hi,
I apologize for the late reply, I was very busy finishing the library prep's in time.
We ended up doing 8 cycles for the higher concentration samples and 12 for lower. The 8 cycles should have...
Forum: Illumina/Solexa 02-25-2020, 12:11 PM
Replies: 3
Views: 1,248
Posted By mdegraaf
Pe150+8(+8?)+150

Hi,
We have prepared a bunch of libraries with the Nextera Flex kit, with the use of the index plate (Nextera™ DNA CD Indexes (96 Indexes, 96 Samples). Now we are sending pooled libraries off to be...
Forum: Illumina/Solexa 02-12-2020, 10:02 AM
Replies: 1
Views: 1,237
Posted By mdegraaf
Question 1/5 Nextera Flex number of cycles

Hi,
We are about to start a fairly large experiment with the Nextera Flex kit in a very limited time. After some testing and reading experiences we have decided to do the kit at 1/5 strength. Now...
Forum: Sample Prep / Library Generation 02-12-2020, 09:50 AM
Replies: 7
Views: 3,331
Posted By mdegraaf
Hi I was wondering if you have answered your own...

Hi I was wondering if you have answered your own question by now. We are doing the Nextera Flex at 1/5 and are wondering about the input and number of cycles.
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