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Forum: Illumina/Solexa 10-25-2010, 08:34 AM
Replies: 8
Views: 3,780
Posted By gford
I titrated the number of Phusion PCR cycles to 17...

I titrated the number of Phusion PCR cycles to 17 for one sample, and 19 on the remaining 4.
Forum: Illumina/Solexa 10-14-2010, 09:21 AM
Replies: 8
Views: 3,780
Posted By gford
re: problem with mRNA multiplex libraries

I've just finished making 300 bp indexed paired end mRNA-seq libraries using these reagents and saw the expected smears on a Bioanalyzer, though they haven't been sequenced yet.
Forum: Illumina/Solexa 04-14-2010, 03:20 PM
Replies: 1
Views: 1,594
Posted By gford
Biotechniques reference

The linked paper describes 600 bp libraries prepared from PCR products:
...
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