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Forum: Metagenomics 10-16-2018, 11:32 PM
Replies: 5
Views: 5,891
Posted By nucacidhunter
I do not know why oligo dT has been used in 1st...

I do not know why oligo dT has been used in 1st strand synthesis. It is possible that dA20 oligo has hybridized to oligo T resulting in creating long stretches of T by joining that has been used as...
Forum: Metagenomics 10-16-2018, 12:02 AM
Replies: 5
Views: 5,891
Posted By nucacidhunter
I do not know how the library has been prepped...

I do not know how the library has been prepped but I notice following:

1- It is stranded (directional) library
2- Probably RNA has been poly adenylated after extraction
3- It contains some short...
Forum: Metagenomics 10-09-2018, 10:53 PM
Replies: 5
Views: 5,891
Posted By nucacidhunter
Could you post full sequence of few reads (both...

Could you post full sequence of few reads (both reads if sequenced paired end).
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