SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 8 of 8
Search took 0.00 seconds.
Search: Posts Made By: MWN
Forum: Illumina/Solexa 11-09-2013, 07:46 AM
Replies: 1
Views: 3,059
Posted By MWN
I like the idea of ssDNA heterduplex as discussed...

I like the idea of ssDNA heterduplex as discussed in
http://seqanswers.com/forums/showthread.php?t=559
Perhaps increasing primer concentrations and/or reducing PCR cycles will help.
Forum: Illumina/Solexa 11-09-2013, 07:07 AM
Replies: 1
Views: 3,059
Posted By MWN
High Mol. Weight Peak from Library on Agilent Bioanalyzer

I made some TruSeq DNA library using Illumina adapter from sonicated FFPE DNA and ran the amplified library after AMPure purification. I have seen the extra high molecular weight peak > 1000 bp. ...
Forum: Illumina/Solexa 09-30-2013, 09:58 PM
Replies: 3
Views: 2,899
Posted By MWN
For V2, I load 11 pM based on Qubit. For V3, 14...

For V2, I load 11 pM based on Qubit. For V3, 14 pM.
Forum: Bioinformatics 03-06-2013, 07:12 PM
Replies: 24
Views: 7,904
Posted By MWN
What additional adapter sequence Haloplex added?...

What additional adapter sequence Haloplex added? Thanks,
Forum: Bioinformatics 03-06-2013, 06:57 PM
Replies: 6
Views: 2,466
Posted By MWN
Any update? How much FFPE DNA do you use?...

Any update?
How much FFPE DNA do you use? Appreciate.
Forum: Bioinformatics 03-06-2013, 06:53 PM
Replies: 4
Views: 4,202
Posted By MWN
How well does Haloplex perform based on your...

How well does Haloplex perform based on your experience? I am thinking about trying it out. However, I am worrying about FFPE DNA input requirement.
Any potential to get copy number information...
Forum: Bioinformatics 03-06-2013, 06:44 PM
Replies: 5
Views: 17,249
Posted By MWN
Could you elaborate on the adapter trimming in...

Could you elaborate on the adapter trimming in Haloplex assay?
Could you include the illumina adapter sequences in the sample sheet? For Nextera, TruSeq or custom adapters, I saw they are trimmed...
Forum: Illumina/Solexa 03-06-2013, 06:37 PM
Replies: 7
Views: 6,241
Posted By MWN
How much DNA input is needed for Haloplex? I...

How much DNA input is needed for Haloplex? I have seen the latest 200 ng input suggestion and even 50 ng input in Agilent slides. Hower at 50 ng, the allele frequences start to show larger...
Showing results 1 to 8 of 8

 


All times are GMT -8. The time now is 02:57 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO