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Forum: Illumina/Solexa 01-15-2014, 11:11 AM
Replies: 17
Views: 13,331
Posted By bnmtthws
great - thanks for the clarification!

great - thanks for the clarification!
Forum: Illumina/Solexa 01-15-2014, 10:05 AM
Replies: 17
Views: 13,331
Posted By bnmtthws
quick question from a relatively recent adopter:...

quick question from a relatively recent adopter: are you implying that the instrument is using different recipes to generate clusters depending on the library type/workflow specified in the sample...
Forum: Sample Prep / Library Generation 09-30-2013, 06:05 AM
Replies: 4
Views: 3,242
Posted By bnmtthws
i hear you - good luck! at this point, i...

i hear you - good luck!

at this point, i don't even bother running out the 1st PCR on a gel - we'll run the results of the 2nd pcr through the qubit and bioanalyzer to check sizing and...
Forum: Sample Prep / Library Generation 09-29-2013, 09:47 AM
Replies: 4
Views: 3,242
Posted By bnmtthws
when we've had similar problems with a barcoded...

when we've had similar problems with a barcoded primer set i've simply performed two rounds of PCR - use the primers without barcodes first, and the reamplify with the barcoded primers.

if you...
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