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Forum: RNA Sequencing 08-29-2014, 04:30 AM
Replies: 15
Views: 20,283
Posted By eastasiasnow
thank you, jeremy, now I am assure what to do.

thank you, jeremy, now I am assure what to do.
Forum: RNA Sequencing 08-28-2014, 07:48 PM
Replies: 15
Views: 20,283
Posted By eastasiasnow
yeah, pooling biological replicate samples will...

yeah, pooling biological replicate samples will lose group variance. but could I use this design to do the following analysis? do people accept this design when I apply it in my paper? if so, what...
Forum: RNA Sequencing 08-28-2014, 01:59 AM
Replies: 15
Views: 20,283
Posted By eastasiasnow
hi Jeremy, have you got the answer of your...

hi Jeremy, have you got the answer of your concern?
Forum: Bioinformatics 01-22-2014, 05:43 AM
Replies: 4
Views: 2,420
Posted By eastasiasnow
@GenoMax Thanks, This should work. I found a...

@GenoMax
Thanks, This should work. I found a script from this post:
http://seqanswers.com/forums/showthread.php?t=4914

I made small changes to that awk script for applying it to the latest...
Forum: Bioinformatics 01-22-2014, 05:28 AM
Replies: 20
Views: 12,168
Posted By eastasiasnow
when running py script on gff file of zea mays...

when running py script on gff file of zea mays v3.21 from ensembl, it popped up this error:


any idea?

for latest ensembl gff file of zea mays AGPv3.21, the awk script should also need some...
Forum: Bioinformatics 01-22-2014, 12:59 AM
Replies: 4
Views: 2,420
Posted By eastasiasnow
yeah, thanks. I got nearly all my necessary...

yeah, thanks. I got nearly all my necessary features.



As for "feature length", I am a little confused. Your suggestion above is ok for "gene feature length", but what about "protein_coding...
Forum: Bioinformatics 01-14-2014, 11:10 PM
Replies: 23
Views: 27,685
Posted By eastasiasnow
one more thing to share tophat2 2.0.10 still...

one more thing to share

tophat2 2.0.10 still doesn't recognize gzipped file in -G option, so do uncompress the gtf file before you run the command. Or you will be confronted with warning "opHat...
Forum: Introductions 01-14-2014, 10:57 PM
Replies: 0
Views: 553
Posted By eastasiasnow
Wink hello from China

hi, good to say something to everyone.

the forum helps me a lot, thanks, although i just registered in. actually i did try to register a year ago, but my old email address is banned by the admin...
Showing results 1 to 8 of 8

 


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