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Forum: Bioinformatics 02-09-2014, 02:43 PM
Replies: 9
Views: 6,467
Posted By GenoMax
If the multiple samples are from the same region,...

If the multiple samples are from the same region, that is not going to help with the low nucleotide diversity problem. You may want to consider deliberately under-loading the library and/or adding a...
Forum: Bioinformatics 02-08-2014, 03:47 PM
Replies: 9
Views: 6,467
Posted By GenoMax
Did this run have a phiX spike? What was the % of...

Did this run have a phiX spike? What was the % of the spike, if it was there? Was this a V2 or V3 chemistry run and what was the cluster concentration?

It is possible that because of the small...
Forum: Bioinformatics 02-08-2014, 03:11 AM
Replies: 9
Views: 6,467
Posted By GenoMax
Have you looked at the quality score distribution...

Have you looked at the quality score distribution of the reads? Was that normal?
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