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Forum: RNA Sequencing 02-19-2014, 02:33 PM
Replies: 2
Views: 1,828
Posted By MAdkisson
Thanks! I'll check that out

Thanks! I'll check that out
Forum: RNA Sequencing 02-12-2014, 01:03 PM
Replies: 2
Views: 1,828
Posted By MAdkisson
Wang, 'Low Cost Library Construction...': Low Concentration

Hi All

I'm using the updated Wang et al Library construction protocol (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026426)[Universal adapters and Indexed Primers +...
Forum: Illumina/Solexa 08-14-2013, 03:24 PM
Replies: 6
Views: 16,015
Posted By MAdkisson
Is anyone certain about whether or not Tween-20...

Is anyone certain about whether or not Tween-20 is involved?
Forum: General 07-30-2012, 01:49 PM
Replies: 1
Views: 2,686
Posted By MAdkisson
Sample Cross-Contamination

Hi All

I've been killing myself trying to find info on dealing with sample cross-contamination in RNA-Seq - lots of warnings to take precautions as cross-contamination is a real issue, but nobody...
Forum: Sample Prep / Library Generation 01-09-2012, 10:55 AM
Replies: 13
Views: 4,075
Posted By MAdkisson
Someone did just do the routine cleaning last...

Someone did just do the routine cleaning last week, but again others are not getting anything like this - of the five runs I've done with these same 12 samples, all giving crap results, someone...
Forum: Sample Prep / Library Generation 01-09-2012, 10:33 AM
Replies: 13
Views: 4,075
Posted By MAdkisson
I decom the electrodes with RNase-Away and then...

I decom the electrodes with RNase-Away and then RNase-free water before starting everytime
Forum: Sample Prep / Library Generation 01-09-2012, 10:32 AM
Replies: 13
Views: 4,075
Posted By MAdkisson
Oh, also, i should mention that in the past, when...

Oh, also, i should mention that in the past, when i have 'troubleshot' this issue, the same samples that were giving me problems would then give often time excellent results. I've also added positive...
Forum: Sample Prep / Library Generation 01-09-2012, 10:29 AM
Replies: 13
Views: 4,075
Posted By MAdkisson
Hi guys I heat denature everything before i...

Hi guys

I heat denature everything before i run. The ladder was changed up in the middle of these attempts. I've added another run, showing the exact same 12 samples, with the new ladder. Same...
Forum: Sample Prep / Library Generation 01-05-2012, 10:31 AM
Replies: 13
Views: 4,075
Posted By MAdkisson
... also, check out the ladder read - even those...

... also, check out the ladder read - even those come out all wonky and never in the same way between chip runs
Forum: Sample Prep / Library Generation 01-04-2012, 03:47 PM
Replies: 13
Views: 4,075
Posted By MAdkisson
Isolating with a modified RNeasy protocol. Have...

Isolating with a modified RNeasy protocol. Have optimized for purity. Also, samples that give these results will then give very good reads - even high RIN values - once I 'fixed' the issue in the...
Forum: Sample Prep / Library Generation 01-04-2012, 02:05 PM
Replies: 13
Views: 4,075
Posted By MAdkisson
Exclamation Wacky BioAnalyzer Results

Hi All

Has anybody ever seen anything like these?
I'm asking this question in as many forums as possible since nobody around me seems to have any clue what is going on - even the techs at Agilent...
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