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Search: Posts Made By: RayLangley
Forum: Illumina/Solexa 01-05-2012, 09:21 AM
Replies: 4
Views: 3,659
Posted By RayLangley
I recently used on lung RNA from cyno maqaques...

I recently used on lung RNA from cyno maqaques samples and it worked great.
Forum: Sample Prep / Library Generation 06-20-2011, 10:46 AM
Replies: 3
Views: 3,782
Posted By RayLangley
Hi James, I haven't tried expanding the...

Hi James,

I haven't tried expanding the TruSeq kits but I did do it with the older kits.

I would prefer keep the input the same. My suggestion is try 1/2 of the master mixes initially and...
Forum: Introductions 06-20-2011, 08:56 AM
Replies: 2
Views: 1,428
Posted By RayLangley
I have not seen any differences. The new kit is a...

I have not seen any differences. The new kit is a lot easier to use than the old kits.

Since we are not using gel selection any more my average peak is around 300 BP. You get a lagging tail that I...
Forum: Introductions 06-17-2011, 01:13 PM
Replies: 2
Views: 1,428
Posted By RayLangley
RE: Geeting from Ray L

Just wanted to say Hi. I have been working with Next-gen sequencing since 2008 as a post doc at NCGR in Santa Fe. I am currently and ARS at LRRI and an ARSe in life ;-). I have numerous pubs with...
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