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Forum: Bioinformatics 01-09-2014, 02:53 AM
Replies: 15
Views: 9,074
Posted By swebb
We have added RNA spike ins to give us the...

We have added RNA spike ins to give us the ability to check for sequencing bias, look at lower limits of detection and hopefully to aid normalisation of transcript abundance. Most of our RNA-seq...
Forum: Bioinformatics 01-07-2014, 06:54 AM
Replies: 15
Views: 9,074
Posted By swebb
DESeq analysis with ERCC RNA spike ins

Hi, I want to perform differential expression analyses of multiple RNA-seq samples using DESeq. We have included ERCC spike in controls and wish to use these to normalise the count data.

I have...
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