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Forum: De novo discovery 03-16-2011, 04:34 AM
Replies: 16
Views: 13,722
Posted By Thorondor
it is really hard to guess. You trimmed all...

it is really hard to guess.

You trimmed all reads to the same length of 50bp?
What total coverage for your transcriptome do you expect with this 31405877 reads. Only option you have left so far...
Forum: De novo discovery 03-15-2011, 01:39 AM
Replies: 16
Views: 13,722
Posted By Thorondor
woah, old thread. ;-) how long are you...

woah, old thread. ;-)

how long are you reads? did you trim before running velvet and oases? which kmers you choose, take care that you do not choose a higher kmer than your minimum read length....
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