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Forum: Sample Prep / Library Generation 04-15-2015, 09:44 AM
Replies: 33
Views: 26,878
Posted By slees
how to make good libraries from <1ng DNA using the Nextera kit

Hi Simone78, I was wondering if your paper has been published? I'm still interested in how to make good libraries from <1ng DNA using the Nextera kit. Thanks!
Forum: Sample Prep / Library Generation 02-10-2014, 07:39 PM
Replies: 3
Views: 3,376
Posted By slees
Thanks luc! The information you provided is very...

Thanks luc! The information you provided is very useful, there is actually a chart on Rubicon Genomics website:
http://rubicongenomics.com/applications/dna-seq/
Forum: Sample Prep / Library Generation 02-10-2014, 02:42 PM
Replies: 3
Views: 3,376
Posted By slees
Question Clontech Low Input Library Prep Kit

Hello,

Anyone used Clontech Low Input Library Prep Kit (Cat. No. 634947)? It is suggested for the lib prep following cDNA synthesis/amplification with the SMARTer RNA-seq kit. But I think it can...
Forum: Sample Prep / Library Generation 01-16-2014, 11:45 AM
Replies: 1
Views: 3,227
Posted By slees
How to fill the gap after tagmentation? Can I use Klenow fragment?

I am trying to making Illumina sequencing library using transposon based method to add adaptor. However, instead of using the adaptor used in Nexera kits, I would like to use the Y-shaped adaptor....
Forum: Sample Prep / Library Generation 10-08-2013, 08:54 AM
Replies: 33
Views: 26,878
Posted By slees
Thumbs up Thanks Simone78. Good luck with your paper! And...

Thanks Simone78. Good luck with your paper! And would you please let me know when it is published?
Forum: Sample Prep / Library Generation 10-03-2013, 06:30 PM
Replies: 33
Views: 26,878
Posted By slees
Thanks Simone78. It will be great if you could...

Thanks Simone78. It will be great if you could also tell us something you have found about how the ionic strength of the final solution affects the reaction.
Forum: Sample Prep / Library Generation 10-03-2013, 09:05 AM
Replies: 33
Views: 26,878
Posted By slees
Good point. Thanks kccchan!

Good point. Thanks kccchan!
Forum: Sample Prep / Library Generation 10-02-2013, 07:32 PM
Replies: 33
Views: 26,878
Posted By slees
Thumbs up I am also curious about this. Also, why does...

I am also curious about this.
Also, why does Illumina suggest to use Nextera XT to make libraries only for small genomes? Can we directly use Nextera XT to make libraries for small amount (<1ng) of...
Forum: Bioinformatics 01-29-2013, 06:36 PM
Replies: 3
Views: 5,727
Posted By slees
worked

Thank you frozenlyse!

"ln -s /usr/lib/x86_64-linux-gnu/libbz2.so /usr/lib/libbz2.so" solved this problem!

However, some more errors appeared after this. After installing some Perl Modules and...
Forum: Bioinformatics 01-29-2013, 01:46 PM
Replies: 3
Views: 5,727
Posted By slees
Bcl2Fastq 1.8.3 install error on Ubuntu 12.04LTS: "No support for bzip2 compression"

Hi,

I was trying to install the CASAVA Bcl2Fastq 1.8.3 package on my Ubuntu Server 12.04LTS. First, I encountered the same "No support for gzip compression" discussed in the following thread:...
Forum: Bioinformatics 01-29-2013, 01:34 PM
Replies: 29
Views: 29,455
Posted By slees
CASAVA 1.8.3 install error on Ubuntu 12.04LTS: "No support for bzip2 compression"

Hi,

I was trying to install the CASAVA Bcl2Fastq 1.8.3 package on my Ubuntu Server 12.04LTS. First, I encountered the same "No support for gzip compression" discussed in this thread, and the...
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