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Forum: SOLiD 07-22-2009, 09:04 AM
Replies: 2
Views: 3,295
Posted By yamayaya
GC bias in Solid system?

Does anybody know the GC bias problem when using solid system for more complicated genome sequencing. e.g. mammal?

Is there any published paper talking about the substantial bias caused by GC...
Forum: Illumina/Solexa 11-08-2008, 05:06 PM
Replies: 5
Views: 2,428
Posted By yamayaya
Thank you very much for kind help. Yes,...

Thank you very much for kind help.

Yes, we have GA pipeline 1.01 on hand. So you mean, if we use eland for 32bp analysis, it will only look at first 32bp, but if eland_extended was employed,...
Forum: Illumina/Solexa 11-08-2008, 05:34 AM
Replies: 4
Views: 3,079
Posted By yamayaya
Thanks. I also have read that paper, but they...

Thanks. I also have read that paper, but they deal with beta genome etc.
Forum: Illumina/Solexa 11-08-2008, 01:58 AM
Replies: 4
Views: 3,079
Posted By yamayaya
GC content

It seems that solexa prefer to sequencing higher GC content region. Does anybody face to this problem?
Forum: Illumina/Solexa 11-05-2008, 09:36 PM
Replies: 5
Views: 2,428
Posted By yamayaya
Question 32bp?

Hi, do anybody have ideas about "32bp" in solexa system?

In user guider, it says that 32 is the max ELAND_READ_LENGTH and it is a seed for sequence reads longer than 32bp.

So, I am wondering...
Forum: Illumina/Solexa 03-19-2008, 10:47 PM
Replies: 1
Views: 2,863
Posted By yamayaya
Smile the filters and syringes for solexa?

Have someone know how many kinds of filters and syringes used in solexa preparation, and what function of these stuff?
Thank you~!
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