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Forum: Bioinformatics 11-13-2013, 07:35 PM
Replies: 0
Views: 1,465
Posted By aka
looking for help for NCBI primer blast

Hey all, I found the NCBI primer blast can only estimate PCR product size with primers less than 36. If I wanted to estimate the product size with longer primers (example: illumina fusion primers),...
Forum: Illumina/Solexa 11-13-2013, 07:29 PM
Replies: 10
Views: 5,921
Posted By aka
Long primers for illumina sequencing

Hello, I was trying to run a pcr for downstream illumina sequencing; and the primers are specifically designed for this purpose (with Illumina adapters and barcodes), so the primers end up been very...
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