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Forum: Bioinformatics 08-28-2015, 02:06 AM
Replies: 0
Views: 910
Posted By dep
autoAug.pl cannot start Launch_PASA_pipeline

Dear Colleagues!

Could someone advise me on Augustus?
I am trying to train Augustus for my fungus species using RNA-seq data. I believe I have installed all the programs it requires, but when I...
Forum: Bioinformatics 08-12-2015, 05:06 AM
Replies: 1
Views: 2,540
Posted By dep
bam2hints problem

Hello All!

I am trying to use BRAKER1 pipeline for a fungus genome annotation, but I experience problems with bam2hints program that is part of the Augustus package. When the pipeline comes to...
Forum: Illumina/Solexa 04-22-2015, 08:23 AM
Replies: 6
Views: 1,409
Posted By dep
OK, thank you for assistance!

OK, thank you for assistance!
Forum: Illumina/Solexa 04-22-2015, 08:09 AM
Replies: 6
Views: 1,409
Posted By dep
Yes, I mean the same. Many Ns there.

Yes, I mean the same. Many Ns there.
Forum: Illumina/Solexa 04-22-2015, 07:03 AM
Replies: 6
Views: 1,409
Posted By dep
Thank you for the reply! Yes, I use...

Thank you for the reply!

Yes, I use bcl2fastq and there are many more Ns in read 2. So, I guess its lost?
Forum: Illumina/Solexa 04-21-2015, 04:35 AM
Replies: 6
Views: 1,409
Posted By dep
Failed second index. Any chances to get some data?

Dear colleagues!
We have conducted a HiSeq 2500 dual index paired end run using TruSeq SBS Kit v3 - HS (200-cycles) chemistry and TruSeq PE Cluster Kit v3 - cBot HS cluster generation kit for...
Forum: Introductions 04-21-2015, 04:24 AM
Replies: 0
Views: 722
Posted By dep
dep intro

Hello everybody!

My name is Dmitrii, I work as a sequencing specialist. I have some experience with 454 GS Junior and Ion Torrent PGM and I have just started working with illumina HiSeq. I hope...
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